Integration of indocyanine green analogs as near-infrared fluorescent carrier for precise imaging-guided gene delivery

Codelivery of diagnostic probes and therapeutic molecules often suffers from intrinsic complexity and premature leakage from or degradation of the nanocarrier. Inspired by the "Y" shape of indocyanine green (ICG), the dye is integrated in an amphiphilic lipopeptide (RNF). The hydrophilic segment is composed of arginine-rich dendritic peptides, while cyanine dyes are modified with two long carbon chains and employed as the hydrophobic moiety. They are linked through a disulfide linkage to improve the responsivity in the tumor microenvironment. After formulation with other lipopeptides at an optimized ratio, the theranostic system (RNS-2) forms lipid-based nanoparticles with slight positive zeta potential enabling efficient condensation of DNA. The RNS-2 displays glutathione responded gene release, activatable fluorescence recovery, and up to sevenfold higher in vitro transfection than Lipofectamine 2000. Compared with a Cy3 and Cy5 labeled fluorescence resonance energy transfer indicator for gene release, the "turn-on" indocyanine green analogs exhibit longer emission wavelength and better positive correlation with the dynamic processes of gene delivery. More importantly, the RNS-2 system enables efficient near infrared imaging guided gene transfer in tumor-bearing mice and thus provides more precise and accurate information on location of the cargo gene and synthesized carriers.FWN – Publicaties zonder aanstelling Universiteit Leide

A nitroreductase and glutathione responsive nanoplatform for integration of gene delivery and near-infrared fluorescence imaging

A novel platform rationally integrating indocyanine green analogues and an arginine-rich dendritic peptide with both nitroreductase (NTR) and glutathione (GSH) reduction responsive linkers was developed. This multifunctional platform can enable selective and efficient gene delivery and specific turn-on fluorescence imaging in tumors.Drug Delivery Technolog

Effects of fertilizer application rate and planting density on photosynthetic characteristics, yield and yield components in waxy wheat

Waxy wheat (Triticum aestivum L.) is grown throughout the world for its specific quality. Fertilization and planting density are two crucial factors that affect waxy wheat yield and photosynthetic capacity. The objectives of the research were to determine the effects of fertilization and planting density on photosynthetic characteristics, yield, and yield components of waxy wheat, including Yield, SSR, TGW, GNPP, GWPP, PH, HI, Pn, Gs, Ci, E and WUE using the method of field experiment, in which there were three levels (150, 300, and 450 kg ha−1) of fertilizer application rate and three levels (1.35, 1.8, and 2.25 × 106 plants ha−1) of planting density. The results suggested that photosynthetic characteristics, yield, and yield components had close relationship with fertilization levels and planting density. Under the same plant density, with the increase of fertilization, Yield, SSR, TGW, GNPP, GWPP, HI, Pn, Gs, E and WUE increased and then decreased, PH increased, but Ci decreased. Under the same fertilization, with the increase of plant density, Yield, SSR, TGW, GNPP, GWPP, HI increased and then decreased, PH, Pn, Gs and E increased, PH and WUE declined. The results also showed that F2 (300 kg ha−1) and D2 (1.8 × 106 plants ha−1) was a better match in this experiment, which could obtain a higher grain yield 4961.61 kg ha−1. Consequently, this combination of fertilizer application rate and plant densities are useful to get high yield of waxy wheat

Spin-filtering and charge- and spin-switching effects in a quantum wire with periodically attached stubs

Spin-dependent electron transport in a periodically stubbed quantum wire in the presence of Rashba spin-orbit interaction (SOI) is studied via the nonequilibrium Green's function method combined with the Landauer-Buttiker formalism. The coexistence of spin filtering, charge and spin switching are found in the considered system. The mechanism of these transport properties is revealed by analyzing the total charge density and spin-polarized density distributions in the stubbed quantum wire. Furthermore, periodic spin-density islands with high polarization are also found inside the stubs, owing to the interaction between the charge density islands and the Rashba SOI-induced effective magnetic field. The proposed nanostructure may be utilized to devise an all-electrical multifunctional spintronic device.Comment: 4 pages, 4 figure

Measurements of J/psi Decays into 2(pi+pi-)eta and 3(pi+pi-)eta

Based on a sample of 5.8X 10^7 J/psi events taken with the BESII detector, the branching fractions of J/psi--> 2(pi+pi-)eta and J/psi-->3(pi+pi-)eta are measured for the first time to be (2.26+-0.08+-0.27)X10^{-3} and (7.24+-0.96+-1.11)X10^{-4}, respectively.Comment: 11 pages, 6 figure

BESII Detector Simulation

A Monte Carlo program based on Geant3 has been developed for BESII detector simulation. The organization of the program is outlined, and the digitization procedure for simulating the response of various sub-detectors is described. Comparisons with data show that the performance of the program is generally satisfactory.Comment: 17 pages, 14 figures, uses elsart.cls, to be submitted to NIM

Measurement of branching fractions for the inclusive Cabibbo-favored ~K*0(892) and Cabibbo-suppressed K*0(892) decays of neutral and charged D mesons

The branching fractions for the inclusive Cabibbo-favored ~K*0 and Cabibbo-suppressed K*0 decays of D mesons are measured based on a data sample of 33 pb-1 collected at and around the center-of-mass energy of 3.773 GeV with the BES-II detector at the BEPC collider. The branching fractions for the decays D+(0) -> ~K*0(892)X and D0 -> K*0(892)X are determined to be BF(D0 -> \~K*0X) = (8.7 +/- 4.0 +/- 1.2)%, BF(D+ -> ~K*0X) = (23.2 +/- 4.5 +/- 3.0)% and BF(D0 -> K*0X) = (2.8 +/- 1.2 +/- 0.4)%. An upper limit on the branching fraction at 90% C.L. for the decay D+ -> K*0(892)X is set to be BF(D+ -> K*0X) < 6.6%

Study of J/ψ→ωK+K−J/\psi \to \omega K^+K^-

New data are presented on $J/\psi \to \omega K^+K^-$ from a sample of 58M $J/\psi$ events in the upgraded BES II detector at the BEPC. There is a conspicuous signal for $f_0(1710) \to K^+K^-$ and a peak at higher mass which may be fitted with $f_2(2150) \to K\bar K$. From a combined analysis with $\omega \pi ^+ \pi ^-$ data, the branching ratio $BR(f_0(1710)\to\pi\pi)/BR(f_0(1710) \to K\bar K)$ is $< 0.11$ at the 95% confidence level.Comment: 11 pages, 5 figures. Submitted to Phys. Lett.

Search for the Rare Decays J/Psi --> Ds- e+ nu_e, J/Psi --> D- e+ nu_e, and J/Psi --> D0bar e+ e-

We report on a search for the decays J/Psi --> Ds- e+ nu_e + c.c., J/Psi --> D- e+ nu_e + c.c., and J/Psi --> D0bar e+ e- + c.c. in a sample of 5.8 * 10^7 J/Psi events collected with the BESII detector at the BEPC. No excess of signal above background is observed, and 90% confidence level upper limits on the branching fractions are set: B(J/Psi --> Ds- e+ nu_e + c.c.)<4.8*10^-5, B(J/Psi --> D- e+ nu_e + c.c.) D0bar e+ e- + c.c.)<1.1*10^-5Comment: 10 pages, 4 figure

Measurements of Cabibbo Suppressed Hadronic Decay Fractions of Charmed D0 and D+ Mesons

Using data collected with the BESII detector at $e^{+}e^{-}$ storage ring Beijing Electron Positron Collider, the measurements of relative branching fractions for seven Cabibbo suppressed hadronic weak decays $D^0 \to K^- K^+$, $\pi^+ \pi^-$, $K^- K^+ \pi^+ \pi^-$ and $\pi^+ \pi^+ \pi^- \pi^-$, $D^+ \to \bar{K^0} K^+$, $K^- K^+ \pi^+$ and $\pi^- \pi^+ \pi^+$ are presented.Comment: 11 pages, 5 figure